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l glutamine  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science l glutamine
    L Glutamine, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/l+glutamine/L-Glutamine/pm42888217-76-26-28
    Average 96 stars, based on 623 article reviews
    l glutamine - by Bioz Stars, 2026-11
    96/100 stars

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    Cell Culture:

    Article Title: Cardiomyocyte-enriched OTUD5 alleviates pathological myocardial hypertrophy by deubiquitinating STAT3 and modulating the STAT3-PGC-1α axis.
    Article Snippet: NIH/3T3 cells (Cell Bank of the Chinese Academy of Sciences) were cultured in high-glucose DMEM (Gibco) containing 10% FBS (R223-00, Vazyme) in a humidified incubator at 37 °C and 5% CO2. .. HL-1 cells (Cell Bank of the Chinese Academy of Sciences) were cultured in Claycomb medium (51800C, Sigma) supplemented with norepinephrine (A0937, Sigma), FBS, and 4 mM L-glutamine (G0200, Solarbio). ..

    Article Title: Mitophagy promotes lung repair and regeneration by restoring epithelial metabolic fitness.
    Article Snippet: .. Cell line culture MLE12 cells (ATCC, CRL-2110) were cultured in HITES medium (Dulbecco's:Ham's F12, 50:50 mix supplemented with 2% FBS (Ausbian, VS500T), 0.005 mg/mL insulin (Tocris, 3435/10), 0.01 mg/mL transferrin (Roche, 10652202001), 30 nM sodium selenite (Sigma-Aldrich, S9133), 10 nM hydrocortisone (MCE, HY-N0583), 10 nM β-estradiol (Sigma-Aldrich, E2758), and 2 mM L-glutamine (Solarbio, G0200) at 37°C with 5% CO2. .. Cell line culture MLE12 cells (ATCC, CRL-2110) were cultured in HITES medium (Dulbecco's:Ham's F12, 50:50 mix supplemented with 2% FBS (Ausbian, VS500T), 0.005 mg/mL insulin (Tocris, 3435/10), 0.01 mg/mL transferrin (Roche, 10652202001), 30 nM sodium selenite (Sigma-Aldrich, S9133), 10 nM hydrocortisone (MCE, HY-N0583), 10 nM β-estradiol (Sigma-Aldrich, E2758), and 2 mM L-glutamine (Solarbio, G0200) at 37°C with 5% CO2.

    Article Title: Multi-omics classification of acute myeloid leukemia guides drug combinations to overcome Venetoclax resistance
    Article Snippet: MOLM13, M07e, U937, HEL, and OCI-AML3 cell lines were cultured in suspension using RPMI-1640 medium (HyClone) supplemented with 10% fetal bovine serum (FBS; Newzerum). .. Kasumi-1 cells were cultured in RPMI-1640 medium containing 20% FBS, 1% sodium pyruvate (100 mM, Solarbio), and 1% L-glutamine (200 mM, Solarbio). ..

    Article Title: Mitophagy promotes lung repair and regeneration by restoring epithelial metabolic fitness.
    Article Snippet: .. MLE12 cells (ATCC, CRL-2110) were cultured in HITES medium (Dulbecco's:Ham's F12, 50:50 mix supplemented with 2% FBS (Ausbian, VS500T), 0.005 mg/mL insulin (Tocris, 3435/10), 0.01 mg/mL transferrin (Roche, 10652202001), 30 nM sodium selenite (Sigma-Aldrich, S9133), 10 nM hydrocortisone (MCE, HY-N0583), 10 nM β-estradiol (Sigma-Aldrich, E2758), and 2 mM L-glutamine (Solarbio, G0200) at 37°C with 5% CO2. ..

    Article Title: Deubiquitinating enzyme MINDY2 regulates TNF-α-induced cell death by targeting RIPK1.
    Article Snippet: L929 (Procell, CL-0137) was purchased from Procell. .. Cells were cultured in DMEM (Sangon, China) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin (NCM, China),1 mM L-Glutamine (Solarbio, China) and 1 mM Sodium Pyruvate (Solarbio, China) at 37◦C under 5% CO2. .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-RIPK1 Cell Signaling Technology Cat#3493; RRID: AB_ 37993588 Rabbit Anti-RIPK3 Cell Signaling Technology Cat#15828; RRID: AB_2722663 Rat Anti-MLKL Millipore Cat#MABC604; RRID: AB_2820284 Rabbit Anti-phospho-RIP1 S166 Cell Signaling Technology Cat#31122S; RRID: AB_2799000 Rabbit Anti-phospho-RIPK3 T231/S232 Cell Signaling Technology Cat#91702; RRID: AB_2937060 Rabbit Anti-phospho-MLKL S345 Cell Signaling Technology Cat#37333; RRID: AB_2799112 Anti-MLKL (phospho S345) antibody [EPR9515(2)] Abcam Cat#ab196436; RRID: AB_2687465 Cleaved Caspase-3 (Asp175) Antibody Cell Signaling Technology Cat#9661; RRID: AB_2341188 Monoclonal ANTI-FLAG M2 antibody produced in mouse Thermo Fisher Scientific Cat#F1804; RRID: AB_262044 Rabbit Anti-DYKDDDDK tag Proteintech Cat#20543-1-AP; RRID: AB_11232216 HA tag antibody Proteintech Cat#51064-2-AP; RRID: AB_11042321 ACTB Monoclonal Antibody Abclonal Cat#AC026; RRID: AB_2768234 GAPDH Monoclonal Antibody Abclonal Cat#AC033; RRID: AB_2769570 Myc-Tag Rabbit mAb Abclonal Cat#AE070; RRID: AB_2863795 IκBα Rabbit mAb Abclonal Cat#A19714; RRID: AB_2862749 Phosphorylated IkBa (Ser32) Cell Signaling Technology Cat#2859; RRID:AB_561111 p38 MAPK (D13E1) XP Rabbit mAb Cell Signaling Technology Cat#8690T; RRID: AB_10999090 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP(tm) Rabbit mAb Cell Signaling Technology Cat#4511; RRID: AB_2139682 JNK Cell Signaling Technology Cat#9252, RRID:AB_2250373 Phosphorylated JNK (Thr183/Tyr185) Cell Signaling Technology Cat#4668, RRID:AB_823588 Vimentin Proteintech Cat#10366-1-AP; RRID:AB_2273020 Beta Tubulin antibody Proteintech Cat#10094-1-AP; RRID:AB_2210695 Rabbit-Anti-Lamin B1 Proteintech Cat#12987-1-AP; RRID:AB_2136290 Recombinant Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] Abcam Cat#ab179434; RRID:AB_2895239 Anti-Ubiquitin (linkage-specific K48) antibody Abcam Cat#ab140601; RRID:AB_2783797 M1-linkage Specific Polyubiquitin Rabbit pAb Abclonal Cat#A18200; RRID: AB_2861977 Rabbit Anti-cleaved-caspase-3 Cell Signaling Technology Cat#9661; RRID: AB_2341188 TRADD Polyclonal antibody Proteintech Cat#15468-1-AP; RRID:AB_2209035 Rabbit Anti-caspase-3 Cell Signaling Technology Cat#14220; RRID: AB_2798429 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Cat#TSC-C14 Chemicals, peptides, and recombinant proteins Necrostatin-1 MCE Cat#HY-15760 Recombinant Mouse CSF-1 Abclonal Cat#RP01216 polybrene Beyotime Cat#ST1380 5Z-7-Oxozeaenol MCE Cat#HY-12686 IFNγ MCE Cat#HY-P7071 MG132 MCE Cat#HY-13259 mTNFα Abclonal Cat#RP01071 Z-VAD-FMK, MCE Cat#HY-16658B (Continued on next page) Cell Reports 45, 117134, April 28, 2026 13

    Expressing:

    Article Title: Identification and Functional Characterization of Novel and Recurrent NTRK1 Variants in Chinese Families With Congenital Insensitivity to Pain With Anhidrosis: A Combined Clinical, Genetic, and Functional Study
    Article Snippet: .. HeLa and HEK293T cells, which exhibit no endogenous NTRK1 expression and are widely used as efficient models for exogenous gene expression [ , ], were employed for functional analysis of the identified variants and routinely maintained in DMEM medium (Gibco, C11995500BT) supplemented with 10% FBS (vivacell, C04001 ), 1% penicillin–streptomycin (Gibco, 15140122), and 1% L‐glutamine (Solarbio, G0200). ..

    Gene Expression:

    Article Title: Identification and Functional Characterization of Novel and Recurrent NTRK1 Variants in Chinese Families With Congenital Insensitivity to Pain With Anhidrosis: A Combined Clinical, Genetic, and Functional Study
    Article Snippet: .. HeLa and HEK293T cells, which exhibit no endogenous NTRK1 expression and are widely used as efficient models for exogenous gene expression [ , ], were employed for functional analysis of the identified variants and routinely maintained in DMEM medium (Gibco, C11995500BT) supplemented with 10% FBS (vivacell, C04001 ), 1% penicillin–streptomycin (Gibco, 15140122), and 1% L‐glutamine (Solarbio, G0200). ..

    Functional Assay:

    Article Title: Identification and Functional Characterization of Novel and Recurrent NTRK1 Variants in Chinese Families With Congenital Insensitivity to Pain With Anhidrosis: A Combined Clinical, Genetic, and Functional Study
    Article Snippet: .. HeLa and HEK293T cells, which exhibit no endogenous NTRK1 expression and are widely used as efficient models for exogenous gene expression [ , ], were employed for functional analysis of the identified variants and routinely maintained in DMEM medium (Gibco, C11995500BT) supplemented with 10% FBS (vivacell, C04001 ), 1% penicillin–streptomycin (Gibco, 15140122), and 1% L‐glutamine (Solarbio, G0200). ..



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